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Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze regions of interest down to single cells to obtain results that are reproducible, and specific. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis. This references list is monthly updated and presents current papers in the major application fields for laser microdissection. |
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Otsubo T, Okamura T, Hagiwara T, Ishizaka Y, Dohi T, Kawamura YI
PLoS One. 2015 Feb 24;10(2):e0116072. doi: 10.1371/journal.pone.0116072. eCollection 2015.
Thi-Ngoc Vo D, Nakayama T, Yamamoto H, Mukaisho K, Hattori T and Sugihara H
BMC Medical Genomics 18 Feb 2015, 8:6 doi:10.1186/s12920-015-0080-6
Establishment of a DNA methylation marker to evaluate cancer cell fraction in gastric cancer
Zong L1, Hattori N, Yoda Y, Yamashita S, Takeshima H, Takahashi T, Maeda M, Katai H, Nanjo S, Ando T, Seto Y, Ushijima T
Gastric Cancer. 2015 Feb 13.
MiR-205 and MiR-375 MicroRNA Assays to Distinguish Squamous Cell Carcinoma from Adenocarcinoma in Lung Cancer Biopsies
Patnaik S, Mallick R, Kannisto E, Sharma R, Bshara W, Yendamuri S, Dhillon SS
J Thorac Oncol. 2015 Mar;10(3):446-53. doi: 10.1097/JTO.0000000000000423.
Facial nerve axotomy in mice: a model to study motoneuron response to injury
Olmstead DN, Mesnard-Hoaglin NA, Batka RJ, Haulcomb MM, Miller WM, Jones KJ
J Vis Exp. 2015 Feb 23;(96). doi: 10.3791/52382
A Sertoli cell-specific connexin43 knockout leads to altered interstitial connexin expression and increased Leydig cell numbers
Noelke J, Wistuba J, Damm OS, Fietz D, Gerber J, Gaehle M, Brehm R
Cell Tissue Res. 2015 Feb 19
Proteomics of the Human Endometrial Glandular Epithelium and Stroma from the Proliferative and Secretory Phases of the Menstrual Cycle
Hood BL, Liu B, Alkhas A, Shoji Y, Challa R, Wang G, Ferguson S, Oliver J, Mitchell D, Bateman NW, Zahn CM, Hamilton CA, Payson M, Lessey B, Fazleabas AT, Maxwell GL, Conrads TP, Risinger JI
Biol Reprod. 2015 Feb 18. pii: biolreprod.114.127217.
Immunofluorescence laser micro-dissection of specific nephron segments in the mouse kidney allows targeted downstream proteomic analysis
Micanovic R, Khan S, El-Achkar TM
Physiol Rep. 2015 Feb 12;3(2). pii: e12306. doi: 10.14814/phy2.12306.
A comparison of immunohistochemistry and mass spectrometry for determining the amyloid fibril protein from formalin-fixed biopsy tissue
Gilbertson JA, Theis JD, Vrana JA, Lachmann H, Wechalekar A, Whelan C, Hawkins PN, Dogan A, Gillmore JD
J Clin Pathol. 2015 Jan 30. pii: jclinpath-2014-202722. doi: 10.1136/jclinpath-2014-202722.
Immunofluorescence laser micro-dissection of specific nephron segments in the mouse kidney allows targeted downstream proteomic analysis
Micanovic R, Khan S, El-Achkar TM
Physiol Rep. 2015 Feb 12;3(2). pii: e12306. doi: 10.14814/phy2.12306.
and Diabetic Cats
Öhlund M, Franzen P, Andersson G, Holst BS, Lau J
Journal of Gastroenterology, Pancreatology & Liver Disorders
Role of glutamatergic projections from ventral tegmental area to lateral habenula in aversive conditioning
Root DH, Mejias-Aponte CA, Qi J, Morales M
J Neurosci. 2014 Oct 15;34(42):13906-10. doi: 10.1523/JNEUROSCI.2029-14.2014.
MiR-205 and MiR-375 MicroRNA Assays to Distinguish Squamous Cell Carcinoma from Adenocarcinoma in Lung Cancer Biopsies
Patnaik S, Mallick R, Kannisto E, Sharma R, Bshara W, Yendamuri S, Dhillon SS
J Thorac Oncol. 2015 Mar;10(3):446-53. doi: 10.1097/JTO.0000000000000423.
Retrotransposition of long interspersed nucleotide element-1 is associated with colitis but not tumors in a murine colitic cancer model
Otsubo T, Okamura T, Hagiwara T, Ishizaka Y, Dohi T, Kawamura YI
PLoS One. 2015 Feb 24;10(2):e0116072. doi: 10.1371/journal.pone.0116072. eCollection 2015.
Progression risk assessments of individual non-invasive gastric neoplasms by genomic copy-number profile and mucin phenotype
Thi-Ngoc Vo D, Nakayama T, Yamamoto H, Mukaisho K, Hattori T and Sugihara H
BMC Medical Genomics 18 Feb 2015, 8:6 doi:10.1186/s12920-015-0080-6
Facial nerve axotomy in mice: a model to study motoneuron response to injury
Olmstead DN, Mesnard-Hoaglin NA, Batka RJ, Haulcomb MM, Miller WM, Jones KJ
J Vis Exp. 2015 Feb 23;(96). doi: 10.3791/52382
Increased dopamine D2 receptor activity in the striatum alters the firing pattern of dopamine neurons in the ventral tegmental area
Krabbe S, Duda J, Schiemann J, Poetschke C, Schneider G, Kandel ER, Liss B, Roeper J, Simpson EH
Proc Natl Acad Sci U S A. 2015 Feb 9. pii: 201500450.
Somatostatin, neuronal vulnerability and behavioral emotionality
Lin LC, Sibille E
Mol Psychiatry. 2015 Jan 20. doi: 10.1038/mp.2014.184.
Cancer Biopsies
Patnaik S, Mallick R, Kannisto E, Sharma R, Bshara W, Yendamuri S, Dhillon SS
J Thorac Oncol. 2015 Mar;10(3):446-53. doi: 10.1097/JTO.0000000000000423.
Progression risk assessments of individual non-invasive gastric neoplasms by genomic copy-number profile and mucin phenotype
Thi-Ngoc Vo D, Nakayama T, Yamamoto H, Mukaisho K, Hattori T and Sugihara H
BMC Medical Genomics 18 Feb 2015, 8:6 doi:10.1186/s12920-015-0080-6
Establishment of a DNA methylation marker to evaluate cancer cell fraction in gastric cancer
Zong L1, Hattori N, Yoda Y, Yamashita S, Takeshima H, Takahashi T, Maeda M, Katai H, Nanjo S, Ando T, Seto Y, Ushijima T
Gastric Cancer. 2015 Feb 13.
A Sertoli cell-specific connexin43 knockout leads to altered interstitial connexin expression and increased Leydig cell numbers
Noelke J, Wistuba J, Damm OS, Fietz D, Gerber J, Gaehle M, Brehm R
Cell Tissue Res. 2015 Feb 19
Menstrual Cycle
Hood BL, Liu B, Alkhas A, Shoji Y, Challa R, Wang G, Ferguson S, Oliver J, Mitchell D, Bateman NW, Zahn CM, Hamilton CA, Payson M, Lessey B, Fazleabas AT, Maxwell GL, Conrads TP, Risinger JI
Biol Reprod. 2015 Feb 18. pii: biolreprod.114.127217.
Enthesis fibrocartilage cells originate from a population of Hedgehog-responsive cells modulated by the loading environment
Schwartz AG, Long F, Thomopoulos S
Development. 2015 Jan 1;142(1):196-206. doi: 10.1242/dev.112714.
Redundancy and metabolic function of the glutamine synthetase gene family in poplar
Castro-Rodríguez V, García-Gutiérrez A, Cañas RA, Pascual M, Avila C, Cánovas FM
BMC Plant Biol. 2015 Jan 22;15(1):20.
Plant and fungal gene expression in mycorrhizal protocorms of the orchid Serapias vomeracea colonized by Tulasnella calospora
Balestrini R, Nerva L, Sillo F, Girlanda M, Perotto S
Plant Signaling & Behavior, 2014 Nov 03, DOI: 10.4161/15592324.2014.977707
Differential spatio-temporal expression of carotenoid cleavage dioxygenases regulates apocarotenoid fluxes during AM symbiosis
López-Ráez J, Fernández I, García JM, Berrio E, Bonfante P, Walter MH, Pozo MJ
Plant Science, 2014 Nov 01, DOI: 10.1016/j.plantsci.2014.10.010
STR profiling of epithelial cells identified by X/Y-FISH labelling and laser microdissection using standard and elevated PCR conditions
Lynch L, Gamblin A, Vintiner S, Simons JL
Forensic Sci Int Genet. 2014 Oct 25;16C:1-7. doi: 10.1016/j.fsigen.2014.10.017.
The development of a method of suspension RNA-FISH for forensically relevant epithelial cells using LNA probes
Williams E, Lin MH, Harbison SA, Fleming R
Forensic science international. Genetics 9 December 2013, DOI: 10.1016/j.fsigen.2013.11.007
New RNA methods for the identification of body fluids and cell types
Fleming R, Harbison SA, Lin MH
Forensic Science International: Genetics Supplement Series, 1875-1768
Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.
ionization mass spectrometry
Esaka F, Suzuki D, Magara M
Anal Chem. 2015 Feb 13.
The effect of nutrients on carbon and nitrogen fixation by the UCYN-A-haptophyte symbiosis
Krupke A, Mohr W, LaRoche J, Fuchs BM, Amann RI, Kuypers MM
ISME J. 2014 Dec 23. doi: 10.1038/ismej.2014.253.
Customized patterned substrates for highly versatile correlative light-scanning electron microscopy
Benedetti L, Sogne E, Rodighiero S, Marchesi D, Milani P, Francolini M
Sci Rep. 2014 Nov 13;4:7033. doi: 10.1038/srep07033.
Isolation of single Chlamydia-infected cells using laser microdissection
Podgorny OV, Polina NF, Babenko VV, Karpova IY, Kostryukova ES, Govorun VM, Lazarev VN
J Microbiol Methods. 2014 Dec 26. pii: S0167-7012(14)00367-4. doi: 10.1016/j.mimet.2014.12.018.
Cyclin-Dependent Kinase 6 Is a Chromatin-Bound Cofactor for NF-κB-Dependent Gene Expression
Handschick K, Beuerlein K, Jurida L, Bartkuhn M, Müller H, Soelch J, Weber A, Dittrich-Breiholz O, Schneider H, Scharfe M, Jarek M, Stellzig J, Schmitz ML, Kracht M
Mol Cell. 2013 Dec 31. pii: S1097-2765(13)00870-8. doi: 10.1016/j.molcel.2013.12.002.
Evolutionary comparisons reveal a positional switch for spindle pole oscillations in Caenorhabditis embryos
Riche S, Zouak M, Argoul F, Arneodo A, Pecreaux J, Delattre M
J Cell Biol. 2013 May 20.
