Discover Greater Depths

Multiphoton Microscope Leica TCS SP8 MP

A confocal microscope based on visible light can leave many details invisible. Sometimes the image you need is hidden deep within tissues, which not only scatter light but are damaged by it too.

The excellent optical design of Leica TCS SP8 multiphoton (MP) system makes use of long wavelengths of fully integrated infrared (IR) excitation lasers, the high efficiency of HyD detectors, and an infrared-optimized optical transmission. These allow you to deeply penetrate into the tissue with super-sensitivity and to uncover the finest details of cellular and subcellular processes.

So whether you work with thick organ sections, intact organs or entire live model organisms, let us show you everything the Leica TCS SP8 MP has to offer

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Obtain Excellent Results with Every Sample

Imagine Imaging More Deeply

The thicker the sample, the more deeply light must penetrate. The more scattering occurs, the less information is obtained.

With multiphoton microscopy, you can use the fact that scattering decreases with longer wavelengths to your advantage. Using light in the red and extended IR spectrum ensures limited scattering and deeper penetration.

Offering motorized correction, dedicated IRAPO objectives and super-sensitive HyD detectors, the Leica TCS SP8 MP optimizes three crucial parameters: optical properties, color correction, and sensitivity.

Make a Deep Impact with the Leica TCS SP8 MP

In vivo Thy1-EYFP mouse, z stack. Adult Thy1-EYFP H line mouse, in vivo (cranial window), 800 µm z stack. Courtesy of Dr. Masahiro Fukuda, Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, Japan.

Everything Adapted

The Leica motCORR Motorized Correction Collar

The refractive index (RI) of living cells and their culture medium is close to that of water, making a water immersion objective the best choice. Such objectives are sensitive to RI mismatches caused by variations in coverglass thickness, temperature changes and specimen inhomogeneity. The result is a blurred image and loss of detail in deep tissue.

The Leica motCORR ensures everything is correct and stays corrected. Its motorized correction collar provides an easy, and precise real-time adjustment of the optics to restore optimal imaging in x, y and z dimensions.

Discover the Leica motCORR Objectives

● Leica HC IRAPO L 25x/1.00 W motCORR − optimized for

    multiphoton imaging

● Leica HC FLUOTAR L 25x/1.00 IMM (ne=1.457) motCORR

    VISIR − optimized for CLARITY treated specimens

● Intuitive motCORR control with the LAS X software

Everything Detected

The Leica QUAD Module

The great depths you need for whole organ or live cell imaging make detecting very faint structures a challenge. The emitted light is backscattered by surrounding structures, causing the signal to weaken. And simply adding more light can damage the tissue.

Combining super-sensitive Leica HyD detectors in a Quad adapter module ensures you can see even the faintest structures in deep tissue sections.

Reach New Depths with the QUAD Module

● Explore deeper tissue sections with a new optical adapter, the Quad module

● Configure the Quad module with up to four super-sensitive Leica HyD

   detectors in the RLD (Reflected Light Detection) position

● Obtain the highest quantum efficiency of 45 % with the Leica HyD RLDs

● Choose a modular detector set-up (PMT and/ or HyD) and get the

    super-sensitivity you need with the chance to upgrade later

For technical specification please click here.

The Correct Approach

Experience Apochromatic Correction at a New Level

An Astonishing Revelation in Live Imaging

High-speed imaging and great image quality: This is the challenge. More light for brilliant imaging means live cells suffer from phototoxic effects and bleaching.

The Leica TCS SP8 MP overcomes this. In MP microscopy, two or more photons reach the fluorophore simultaneously, but only in the focal plane. This reduces out-of-focus excitation compared to confocal microscopy, reduces photodamage, and increases cell viability. In addition, the Leica TCS SP8 MP offers high speed and efficient photon collection to catch the fastest kinetics and the faintest signals.

Discover Our Live Imaging Capabilities

A Perfect Working Relationship

The Tandem Scanner

With the Tandem Scanner Leica offers the perfect relationship between a large field of view (FOV) and a full range of scan speeds – the ideal combination for live specimen imaging. If your research covers everything from rapid calcium dynamics in brain slices to whole organ samples, the Leica TCS SP8 MP will be in synergy with you.

All in Time

● 22 mm FOV – the largest of any point scanning systems for scanning in one shot with the patented x2y scanning mirror concept

● Unique combination of FOV and 8 kHz or 12 kHz resonant scanning system with switchable galvanometric mirrors

● Scan at frequencies of up to 12 kHz, resulting in about 40 fps (512 x 512 px) or 428 fps (512 x 16 px)

● Acquire 4D stacks up to 50% faster than comparable systems by combining them with the SuperZ GalvoFlow

● No compromise in resolution, sensitivity or contrast, thanks to high-NA objectives and the sensitive Leica HyD RLDs

Everything Detected

Not only depths, but also speed can make detecting very faint structures in live samples a challenge. If you want to observe fast kinetics, you can increase scan speed, but this means less photons will be collected.

With the Quad module, you can combine up to four super-sensitive Leica HyD detectors to image multiple colors simultaneously. The more sensitive your detector, the brighter your acquired live image. Due to the HyDs´ remarkably low dark noise and their high sensitivity you can reduce laser excitation power, and therefore specimen damage and photobleaching.

Reach New Sensitivity with the QUAD Module

Everything Fixed

See Color Perception in a New Light

Multiple-labeled fluorescence provides insights into localization and interaction. But imaging multiple fluorophores can result in cross-excitation when laser wavelengths are limited.

With the Leica TCS SP8 MP, cutting-edge infrared lasers offer IR excitation even into the far red. And using multiple hybrid detectors systems, multicolor sensitivity is drastically improved.

Perceive the Benefits of Multicolor Experiments with the Leica TCS SP8 MP

Everything Excited

With the Leica TCS SP8 MP you can make full use of the most advanced infrared lasers on the market and take your multiphoton imaging to new depths. Tune gap-free from 680 nm to 1300 nm through the intuitive LAS X software with a range of lasers that reaches from ultra-fast prechirped femtosecond lasers to the Optical Parametric Oscillator (OPO MPX) and InSight DeepSee+.

Make Exciting Discoveries with the Leica SP8 MP

HyD RLD Quad module for four color acquisition.

Everything Detected

Sometimes, observing the localization or dynamics of just one structure provides a limited picture without seeing relationship to its neighbors.

The Quad module improves multicolor super-sensitivity. With up to four Leica HyDs attached, you can fast acquire multicolor and gain insights in localization and interactions from deep within tissues or living specimens.

Reach More Colors with the Quad Module

Everything and More

Label-free Imaging Through Second and Third Harmonic Generation (SHG/THG)

By employing infrared femtosecond lasers, you can see more than just fluorescence. The high energy produced by these short-pulsed IR lasers is sufficient to create another photoelectric effect without the need for labeling. These are SHG and THG, non-linear polarization effects in the illuminated sample.

SHG signals occur from large centrosymmetric structures with spatial order including collagen, myosin or microtubules. THG can be used to detect an interface between materials of different excitability, for example, the outline of cells in embryos, where the cells are separated by water.

See More with the Leica TCS SP8 MP

● Discover more tissue and live cell imaging without using a dye

● Achieve the brightest SHG and THG imaging with super-sensitive HyDs

   and dedicated filters

● Click here to explore the principle of SHG and THG

Zebrafish (gastrulation) RNA injection for Neptune fluorescent protein cytoplasmic staining. THG (in blue). 20x1NA; Insight at 1140 nm. Courtesy of Dr. Nadine Peyrieras, BioEmergences, Gif sur Yvettes, France.

Thy1-YFP mouse brain treated with CLARITY. Animation generated with LAS AF 3D Visualization. Courtesy of Karl Deisseroth and Raju Tomer, Stanford University, Palo Alto, CA, USA.

Let's Get Clarity

Many existing techniques for brain imaging are limited to small regions from thin section, which impedes the study of cellular connection and pathways. With clearing techniques like CLARITY and BABB you can image tissues whole and intact.

The Leica HC Fluotar L 25x/1.0 IMM motCORR VISIR (n_e=1.457) is a specialist immersion objective, specifically designed to maximize results from CLARITY-treated specimens. So, by combining CLARITY with the Leica TCS SP8 MP, you can maximize imaging depth and resolution.

Discover perfect CLARITY

Everything Clear

Optical performance in neuroscience and developmental biology requires a team that works in perfect synergy. By combining the core microscope platform DM6000 CFS with the Leica HCX APO L20x/0.95 IMM objective and BABB immersion medium you can reveal the secrets of unexplored depths.

Shaped for Things to Come

The Leica TCS SP8 MP is configured for your research today but adaptable for tomorrow. Like all Leica Microsystems’ confocal products, the Leica TCS SP8 MP allows you to build and tailor your system upon its easy-to-use core. It can evolve, adapt, and reconfigure to your needs. So, as one discovery leads to another, you can keep the Leica TCS SP8 MP at the very heart of everything you do

Build Upon the Flexible and Easy-to-Use Leica TCS SP8 MP

Mouse diaphragm. Signal from muscle (red), motor neurons (green). Courtesy of R. Rudolf, Karlsruhe Institute of Technology, Germany.

Adapt it to FLIM

The fluorescence lifetime is a measure of how long a fluorophore remains in its excited state before returning to its ground state. FLIM (Fluorescence Lifetime Imaging) lets you display the fluorophore lifetime in every single pixel, adding an extra dimension to your research.

Using the fast Leica HyD RLD detectors for FLIM-FRET (Fluorescent Resonant Energy Transfer) experiments you can easily explore molecular interactions in depth.

Create High-quality FLIM Data From Large Z Stacks

Powerful Performance Made Simple

Performing experiments and analyzing your data shouldn’t be as complex as the science you perform. When designing the LAS X, we focused on just two things: powerful features available for microscopy and an intuitive usability from interface to workflow.

Step-by-Step Guidance with LAS X

Wherever your multiphoton experiments take you, the LAS X software will provide you with powerful analysis options.

Step-by-Step Guidance with LAS X