Chiroptical spectroscopy has become one of most important techniques for the characterization of biomolecules, determination of absolute configuration and stereochemical analysis. Since launching the Model AP-1, our first spectropolarimeter in 1961, JASCO has designed and built the finest in chiroptical instrumentation. Based on JASCO's experience in CD instrumentation over a half century, JASCO proudly introduces the J-1000 Series Circular Dichroism (CD) Spectrometers providing both of unparalleled optical performance and versatile flexibility.
The J-1500 is high-performance CD spectrometer for versatile measurements.
Key Features
- Standard air-cooled 150W Xenon lamp or high-power water-ooled 450W Xenon lamp
- Wide spectral range from vacuum UV to Near-IR (up to 1600 nm as option)
- Standard built-in mercury lamp and optional NIST traceable standard sample for system validation
- High-efficiency purge capability enabling to enhanced vacuum UV measurement
- Extremely low stray light and high S/N ratio providing wide dynamic range
- High-speed scanning up to 10000 nm
- Simultaneous Multi-probe measurements (SMP) with acquisition of up to four data channels
- Flexible design allowing field upgrades for different measurement modes and accessories as applications evolve
- Spectra Manager 2 or Spectra Manager CFR (For FDA regulated labs): 64 bit innovative, cross-platform Spectroscopy Software Suite for data acquisition, analysis and presentation including several methods of secondary structure calculation
Simultaneous Multi-probe measurements
The latest Quad Lock-in Amplifier allows the simultaneous acquisition of up to four data channels including CD, Absorbance, Linear Dichroism (LD), Fluorescence, Fluorescence detected CD (FDCD), Fluorescence detected LD (FDLD), Fluorescence Anisotropy and Optional Rotatory Dispersion (ORD) etc. The following figure shows the multi-probe measurement of Lysozyme showing the simultaneous acquisition of CD, Abs, Ex Fluorescence and Em Fluorescence during a thermal ramping experiment.
Broad range of Measurement Options
Designed as a “Chiroptical Spectroscopy Workbench” the J-1500 offers a wide range of accessories to allow it to be adapted to any application requirements. Temperature ramping, protein folding, enzyme kinetics, DNA/RNA interactions, natural organic chemistry, biochemistry, macromolecules and rapid scanning experiments are all possible.
- Peltier cell holders, single and six-position cell changers
- Micro sampling disk and Capillary jacket for measurement down to a few microliters
- Near-infrared extended detection to 1600nm
- Highly-accurate ORD attachments
- High-sensitivity, artifact-free FDCD attachments
- Total Fluorescence and 90° light scattering
- Fluorescence Excitation/Emission scanning
- Fluorescence Anisotropy, Fluorescence Polarization
- Permanent and electro-magnets for Magnetic Circular Dichroism (MCD)
- Automatic titration system
- 2, 3 and 4-syringe stopped-flow systems
- High-throughput Automated CD system
- LD flow Couette cell holder
- Pressure-resistant high temperature measurement unit
- Solid sampling Diffuse Reflectance/Transmittance CD measurement units
- Liquid N2 Cryostat
- Double-beam UV measurement unit
etc.
Optional Program
- Multivariate protein secondary structure estimation program
- Detatured protein analysis program
- Multi-WL variable temperature measurement program
- Macro command program
- And etc.
