Hepatitis A Virus Detection

The Hepatitis A Virus Detection Kit KHAV allows the detection of hepatitis A virus by real-time RT-PCR, in all types of environmental (water, wastewater, seawater, sludge, air, surface, etc.) and food (shellfish, fruits, vegetables, processed food, etc.) samples. 48 real-time RT-PCR reactions can be performed with each kit. In compliance with ISO/TS15216-1&2 Standard.

•       Utilizes molecular technologies for validated detection of Hepatitis A virus

•       Allows for detection down to 5 hepatitis A copies per reaction

•        Assemble RT-PCR reactions with included master mix, enzyme mix, internal positive control, and negative control

Hepatitis A Virus and Transmission

Hepatitis A virus is a non-enveloped single strand RNA virus. The delay of incubation is around one month. Transmission via the fecal-oral route is the most common. Food samples can be contaminated via food handlers or by the environment. Due to their structural properties, Hepatitis A virus can be resistant to different physical/chemical treatments and food processes. Adsorbed on particles, they can survive in different kinds of environments for long periods of time.
The consumption of contaminated food can lead to human outbreaks. There is no specific curative treatment but a vaccine is available.

Molecular Detection of Hepatitis A

Serological methods have not been effectively adapted for hepatitis E virus detection in environmental or food samples. Detection by cell culture is difficult. Detection by real time RT-PCR, after extraction and purification of the viral RNA, is a method of choice taking into account its speed and its sensitivity