The Norovirus GI Detection Kit KNVGI

allows the detection of genogroup I noroviruses by real-time RT-PCR, in all types of environmental (water, wastewater, seawater, sludge, air, surface, etc.) and food (shellfish, fruits, vegetables, processed food, etc.) samples. 48 real-time RT-PCR reactions can be performed with each kit. In compliance with ISO/TS15216-1&2 Standard.

•       Utilizes molecular technologies for validated detection of norovirus genotypes

•       Allows for detection down to 5 norovirus genome copies per reaction

•       Assemble RT-PCR reactions with included master mix, enzyme mix, internal positive control, and negative and positive controls

Norovirus Genogroup I
Genogroup I noroviruses are non-enveloped, single strand RNA viruses. These human enteric viruses are responsible for most of viral gastroenteritis outbreaks. They are pathogenic for all age groups of the population. These viruses are very infectious; 10 to 100 viral particles are sufficient to get sick. For identification of genogroup I noroviruses use the Norovirus GI Detection Kit.

Norovirus Transmission
Their mode of transmission is the feco-oral route. Food samples can be contaminated via food handlers or by the environment. After excretion in human stool, due to their structural properties, noroviruses can be resistant to different physical and chemical treatment. Adsorbed on particles, noroviruses can survive in different kinds of environments for long periods of time and resist to different food processes.

Molecular Detection of Norovirus
There is no cell culture system presently available for the multiplication of human noroviruses. Detection by ELISA is not recommended due to norovirus genetic diversity and its lack of sensitivity. Molecular techniques (real time RT-PCR) are the methods of choice for norovirus detection after extraction and viral RNA purification from the sample, as described in the ISO/TS15216-1&2 Standard.

Validated for all Norovirus Geneogroup I Genotypes
The Norovirus GI Detection Kit has been validated on all the different genotypes of genogroup I noroviruses. For testing on Food and Environmental matrices only.