Introduction:

This study evaluated the effectiveness of the NovaWAVE for the digestion of beef spiked with TiO2.

Supplies and Reagents:

1) NovaWAVE SA Model* 2) Teflon® 75 ml with Quartz Sleeve Vessels* 3) Teflon® Caps and Safety Pressure Release Caps (pre-set release pressure to 435 psi)* 4) PlasmaPURE HNO3 (70%) 5) PlasmaPURE HF (48%) 6) ICP-OES, Spectroflame FMD-007, Spectro ® * Manufactured by SCP SCIENCE

Sample Preparation Procedure:

The beef was weighed (0.2) on a 4 place analytical balance directly into the 75 ml Teflon vessels. After adding 4:1 HNO₃:HF (5 ml total) of acid and 0.01 g of TiO₂, the samples were digested in the NovaWAVE following the instrument’s heating profile noted below. After cooling, the samples were diluted to 200 ml with water in a volumetric flask. The solutions are clear with no particulates remaining.

NovaWAVE Heating Program:

Analysis:

Conclusion

The sample had to made from 2 components as it is very difficult to mix TiO₂ into ground beef to ensure homogeneity. NovaWAVE work well to digest a sample of meat that has Nano particles in the sample.