The aim of this booklet is to provide the microscopist with a basic explanation of the theory of the microscope sufficient to enable him to understand the reasons behind accepted microscope techniques. It is felt that such an understanding will not only add to his interest in using the microscope, but will help him to work his way out of possible problems that may arise later on when detailed instructions, originally grasped, may have been forgotten. Where possible, mathematical formulae have been avoided in favor of physical or pictorial explanations, as it is felt that such explanations are more easily grasped and better retained than explanations involving mathematics
Id | Content |
1.0 | Introduction |
2.0 | What’s in a Microscope |
3.0 | What Limits the Magnification |
4.0 | Resolving Power |
5.0 | Numerical Aperture |
6.0 | Depth of Focus |
7.0 | Image Aberrations |
8.0 | Aberrations in the Microscope |
9.0 | Apochromatic Objectives |
100. | Types of Eyepieces |
11.0 | Binocular Observation |
12.0 |
The Illumination System |
13.0 |
Condenser Types |
14.0 |
Critical Illumination |
15.0 |
Selective Filters |
16.0 |
Darkfield Illumination |
17.0 |
Polarized Light |
18.0 |
Fluorescence Illumination |
19.0 |
Phase Contrast Microscopy |
20.0 | Concluding Remarks |
Leica Microsystems is a world leader of microscopes that combine high performance and practical design. The educational line offers quality microscopes that withstand everyday student use at an affordable price. Teach beyond the regular microscopy applications with Leica educational products.
Leica DM750
• Focusable or fixed eyepieces • Field of view of 20 mm • 45 degree tube • Wear resistant • Energy saving |
Leica BM E
• Superb, upgradeable Leica E series optic and performance at an excellent price • Tungsten-halogen illumination system provides lamp life of over 2,000 hours, 12 W or a fluorescent system with 10,000 hours life, 7 W • A wide variety of video and photography accessories provide flexibility • Designed to meet or exce electrical requirements |
Leica DM500
• No adjustment needed for parfocality with corrected vision • Large selection for different ergonomic requirements • Easy carrying and lifting onto high shelves • Patented captive thumb-screw for safe rotation of the EZTube™ |
Leica EZ4
• Excellent three-dimensional viewing applications • Enclosed optical system resists damage and tampering • 4:1 zoom capability without losing focus • Effective three-way illumination system: transmitted, reflective or both |
Leica Camera Systems
• Choose from Leica’s multi-purpose camera system or the high performance systems • Add accessories to your microscope to increase learning capabilities
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