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A Shift of Perspective
Inverted Microscope
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Leica DMi8 Advanced Fluorescence Imaging System
Find your individual Leica DMi8 solution dedicated to your application based on our proposal.
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Leica Application Suite X (LAS X) is the imaging and analysis software for live cell imaging from basic documentation to advanced fluorescence imaging, including super-resolution and confocal microscopy.
Learning to use LAS X software for all of your applications is straightforward. Utilizing a workflow-based structure, the software is built to facilitate a quick understanding and use for even very complex analyses. With one software platform for all, training and implementation hurdles are reduced for multi-user and imaging facility environments.
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From Beautiful Images to Quantitative Results
Obtaining qualitative results only is not sufficient. Modern microscopy requires statistical evidence and tools for automated quantification. LAS X offers comprehensive solutions for interactive measurements and wizard-based automated 2D and 3D image analysis. For specialized analysis, it is even possible to load functions and macros from open sources such as ImageJ directly into LAS X. |
Impressive Focus Accuracy
Do you need a fast software auto-focus, but also highly accurate positioning to follow small structures like vesicles over time? Or do you need high resolution three-dimensional images of large data sets?
In both cases, standard microscope focus drives do not work with sufficient accuracy to obtain meaningful data at high resolution. Rather than using a step motor, the Closed Loop Focus drive measures and positions with 20 nm reproducibility over an increased travel range of 12 mm. With this level of precision, 3D imaging is more accurate than ever before. |
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Stay in Focus
Adaptive Focus Control (AFC) actively keeps the focus stable even after changing environmental conditions. The image-based High Speed Autofocus function in LAS X uses independent camera and illumination settings to find the focal plane with a minimal amount of light on the sample. Combine AFC with High-Speed Autofocus to actively find, refocus and reduce time focusing. Not only will your experiment stay in focus, but with minimal phototoxicity unwanted stress on your cells will be reduced. |
High-Speed Fluorescence
Leica Microsystems’ External Filter Wheels for living cell applications are fast, vibration-free, and exceptionally versatile. Their short switchover times are perfect for fast multi-color time-lapse and 3D acquisition. Use Leica Microsystems’ fast filter wheels for excitation, attenuation and emission control to capture cellular events at the speed of life.
Excitation and emission light can be selected in just 24 milliseconds (acquisition speed: 31 fps), and individual excitation colors can be attenuated just as quickly. |
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Cell viability assay. Courtesy of Adrien Eberlin & Marc Koch, IGBMC, Strasbourg, France.
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Brilliant Illumination
Only illuminate your cells when you need to. Fast switching LED based light sources like Lumen Dynamics X-Cite® XLED1 and Lumencor SPECTRA X light engine® as well as the proven Leica EL6000, now with a faster and more precise shutter, minimize phototoxicity during long term experiments. |
The Infinity Port
The Infinity Port provides access into the infinite light path of the microscope, creating outstanding flexibility for fluorescence illumination and manipulation. With this novel design, the microscope allows integration of additional fluorescent light sources and laser systems for advanced applications like FRAP, photo-switching, ablation, optogenetics, and many more. |
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