NanoWizard 4 XP BioScience

The NanoWizard 4 XP BioScience comes with:

• PeakForce Tapping® for easy imaging
• Fast Scanning option with up to 150 lines/sec
• NestedScanner Technology for high-speed imaging of surface structures up to 16.5µm with outstanding resolution and stability
• New tiling functionality for automated mapping of large sample areas
• V7 Software with revolutionary new workflow-based user interface
• DirectOverlay™ 2 software for perfect integration and data correlation with advanced fluorescence microscopy platforms
• Vortis™ 2 controller for high-speed signal processing and lowest noise levels

The HybridStage™ or Motorized Precision Stage transforms experiments by enabling direct access to a large sample area, with automated, motorized movement to selected positions, grids and mapping regions.

Begin with the DirectOverlay 2 optical calibration, and then select a region for optical tiling up to millimeters in size.

Precise motor movements automatically bring the whole sample into view, making it easy to select regions and features for further investigation. A single click navigates from point to point or MultiScan experiments automate a sequence of measurements at selected points.

The images show living Vero cells in cell culture medium at 37°C in the PetriDishHeater™ . [2] – [5] Optical tiling with 5×6 phase contrast images covering a 630µm×450µm region. – Zoom into region scanned with AFM showing 100μm×100μm scan (height range 5μm) and inset 15μm×15μm (height range 2μm) scan topography images using PeakForce Tapping. The feedback correction signal images highlight the surface membrane features, particularly in the zoomed image. Microvilli dominate the center of the cell, with membrane ruffles at the cell boundary.

The images show NanoWizard 4 XP setups on Zeiss LSM 880 confocal microscope with Airyscan [1] , with Upright Fluorescence Microscope (UFM) Kit for tissues or other large samples such as organs [2] , with BioMAT Workstation for high NA optics and Zeiss Axio Imager [3] , with TopviewOptics [4] and on on Olympus with PicoQuant MicroTime 200 STED [5].  

QI™ Advanced, based on real force curves, offers both astounding speed and resolution for applications ranging from single molecules to living cells. The quantitative data allows precise and fast analysis of mechanical or biochemical interactions, e.g., localization of binding sites, directly overlaid with fluorescent labelling and topography with Molecular Recognition Imaging. Advanced batch processing options include multiple models for modulus fitting and can reveal surface topography at zero force with Contact Point Imaging (CPI).

The image shows single cell force spectroscopy measurements using the CellHesion module with an increased z range of 100 µm, showing the detachment force curves of a single A549 cell from fibronectin (FN) and from bovine serum albumin (BSA) coated culture dishes. Note, that the detachment of the cell from fibronectin results in a very large pulling range of 77 µm.