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Vật lính PL APO CS và CS chuyên cho chụp ảnh quét đồng tiêu

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Within the class of apochromats, Leica offers PL APO lenses especially designed to match the highest specifications for confocal scanning (CS). The latest PL APO CS2 series was further improved over the previous CS series. The chromatic correction of the new Leica CS2 objectives is perfect over the whole field of view for precise colocalization of different fluorophores. In addition, numerical aperture and free working distance are pushed to new limits. The design of the Leica CS2 objectives goes hand in hand with the innovative UV optics of the Leica TCS SP8, to give the most stable UV color correction. For aberration-free imaging in aqueous samples such as living cells, Leica Microsystems has developed a series of superior high-resolution water immersion objectives. For the best imaging results, these objectives require a correction collar to adapt the optics to varying coverglass thickness, changing temperature, and specimen inhomogeneity.

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Leica Apochromats

Leica apochromats are objectives for applications with highest specifications in the visual range and beyond, offering field flatness up to 25 mm.

The absolute values of the focus differences for the red wavelength and the blue wavelength to greenwavelength (3 colors) are ≤ 1.0 x depth of field of the objective.

Objective Classes

Objectives are categorized into performance classes on the basis of their transmission, chromatic correction, planarity, and others. From the point of view of the International Organization of Standardization (ISO), there are three groups of objective classes differing in quality of chromatic correction.

Objective Class	Field Flatness	Absolute Value(s) of Focus	Application
Achromats	up to 23 mm	between red and blue wavelength (2 colors) is ≤ 2x depth of field	for standard applications in the visual spectral range
Semi-Apochromats	up to 25 mm	for red wavelength and blue to green wavelength (3 colors) are ≤ 2.5x depth of field	for applications in the visual spectral range with higher specifications
Apochromats	up to 25 mm	for red wavelength and blue to green wavelength (3 colors) are ≤ 1x depth of field	for applications with highest specifications in the visual range and beyond

Depth of field:

Leica Microsystems has aligned all three performance classes of its range of objectives to the needs of its customers and the specific requirements of a wide variety of applications – while offering a convincing price-performance ratio.